Development Of Glycosaminoglycan Scaffold Integrated With Herbal Nanoparticle For Wound Healing

Glycosaminoglycans, the most abundant heteropolysaccharides, have an inevitable role in tissue hydration, cell adhesion, regulation of cell growth and proliferation. Glycosaminoglycanchitosan-gelatin (GCG) scaffold incorporated with Hemigraphis colorata silver nanoparticles was prepared. The study confirmed the physical cross linking of the scaffold using scanning electron microscopy (SEM) and showed potent antibacterial and antioxidant activity. GCG scaffold formed almost uniform pore size even without addition of chemical cross linking agent. MTT Cell Proliferation and invitro scratch wound healing assay proved that the said scaffolds are noncytotoxic, promote cell adhesion and have potent wound healing property. Silver nanoparticles of Hemigraphis colorata with an average size of 23.0nm (as per TEM analysis), integrated in this scaffold enhanced its potential application as burn wound dressing material.GCG scaffold prepared by freezing and lyophilization is soft, spongy and flexible, thus promising its task to advance the burn wound treatment management with release of drug resulting in closure of